Epinephrine (EP), an important neurotransmitter, energizes and speeds up the various body systems and
plays an important role during the time of stress and low blood sugar level. There is a close relation
between the release of epinephrine and smoking. Edge plane pyrolytic graphite electrode modified with
multi-walled carbon nanotubes (MWNTs/EPPGE) has been used as a sensor for the efficient quantitative
determination of epinephrine in body fluids of smokers and nonsmokers in resting stage at physiological
pH 7.2 by using cyclic voltammetry (CV) and square wave voltammetry (SWV). The oxidation of
epinephrine occurred in a well-defined peak having peak potential (Ep) ∼150mV at pH 7.2. The limit of
detection (3/slope) and limit of quantification were found to be 0.15×10−9 and 0.48×10−9Musing proposed
sensor, respectively. The modified electrode was also utilized for the analysis of commercial sample
of epinephrine in order to examine the accuracy of the proposed method. The analytical performance of
the modified electrode has been evaluated for quantification of EP in real samples even in the presence
of common coexisting biomolecules such as uric acid, ascorbic acid, dopamine and norepinephrine. The
voltammetric response of the developed nanosensor towards epinephrine determination in body fluids
is fast, sensitive and selective having desirable reproducibility and stability. A comparison of results with
high performance liquid chromatography (HPLC) shows a good agreement